Viscoelasticity of the leading edge, i.e., the lamellipodium, of a cell is the key property for a deeper understanding
of the active extension of a cell’s leading edge. The fact that the lamellipodium of a cell is very thin (\1000 nm) imparts special
challenges for accurate measurements of its viscoelastic behavior. It requires addressing strong substrate effects and
comparatively high stresses ([1 kPa) on thin samples. We present the method for an atomic force microscopy-based
microrheology that allows us to fully quantify the viscoelastic constants (elastic storage modulus, viscous loss modulus, and the
Poisson ratio) of thin areas of a cell (\1000 nm) as well as those of thick areas. We account for substrate effects by applying
two different models’a model for well-adhered regions (Chen model) and a model for nonadhered regions (Tu model). This
method also provides detailed information about the adhered regions of a cell. The very thin regions relatively near the edge of
NIH 3T3 fibroblasts can be identified by the Chen model as strongly adherent with an elastic strength of ;1.6 6 0.2 kPa and
with an experimentally determined Poisson ratio of ;0.4 to 0.5. Further from the edge of these cells, the adherence decreases,
and the Tu model is effective in evaluating its elastic strength (;0.6 6 0.1 kPa). Thus, our AFM-based microrheology allows us
to correlate two key parameters of cell motility by relating elastic strength and the Poisson ratio to the adhesive state of a cell.
This frequency-dependent measurement allows for the decomposition of the elastic modulus into loss and storage modulus.
Applying this decomposition and Tu’s and Chen’s finite depth models allow us to obtain viscoelastic signatures in a frequency
range from 50 to 300 Hz, showing a rubber plateau-like behavior.