Aim of our study is to use Atomic Force Microscopy (AFM) to measure the spring constant for single bovine serum albumin (BSA) ‘ polyclonal antibody to BSA (Ab-BSA) complex (kcomplex) as a function of pulling off force and complex extension. By the combination of AFM operated in the force-spectroscopy...

We report herein measurements on a novel type of supported lipid films, which we call painted supported membranes (PSM). These membranes are formed in a self-assembly process on alkylated gold films from an organic solution. The formation process was investigated with surface plasmon resonance microscopy....

Since its invention, the atomic force microscope (AFM) has been used to image a wide range of samples, including soft biological materials. Although various imaging modes have been developed in recent years, the images obtained by these techniques are primarily derived from the mechanical interactions...

With AFM related techniques we investigated the specific interaction between avidin and biotin. The contact rupture force (adhesion) between avidin functionalized tips and biotin, which was covalently linked to agarose beads, was measured and characterized. A comprehensive study of the physical properties...

One of the unique features of the atomic force microscope (AFM) is its capacity to measure interactions between tip and sample with high sensitivity and unparalleled spatial resolution. Since the development of methods for the functionalization of the tips, the versatility of the AFM has been expanded...

The change in position of fluorescent beads captured inside the focal volume of optical tweezers is monitored using fluorescence emission induced by two-photon absorption of a continuous-wave Nd:YAG laser ~l51064 nm!. The displacement of a bead due to interactions with its environment leads to a fluorescence...

A new scanning probe microscope, the photonic force microscope (PFM), based on optical tweezers and two-photon absorption processes for biological applications is described. Optical tweezers are used to trap a fluorescent latex bead with a diameter of 200nm in a aqueous solution in all three dimensions....